Thioglycollate broth (NIH & USP): For sterility assays of biological and pharmaceutical products. Broth can be stored for up to 1 month under refrigeration (2/25°C) if properly prepared and protected from light. INOCULATION Incubate the containers inoculated with bacterial strains at 35 ± 2°C for a 18-48 hours. INTERPRETATION Candida albicans ATCC 10231 Good growth, turbidity. Clostridium sporogenes ATCC 19404 Good growth, turbidity. Streptococcus pyogenes ATCC 19615 Good growth, turbidity. Bacteroides fragilis ATCC 25285 Good growth, turbidity. Escherichia coli ATCC 25922 Good growth, turbidity. Bacillus subtilis ATCC 6633 Good growth, turb...